Effects of ADAR2 over-expression or ADAR2 knock-down on AMPA-triggered [Ca2+]
. A: Cortical neurons were transfected with empty vector pcDNA6, pcDNA6 carrying wild-type ADAR2 cDNA, scrambled short hairpin RNA (shCon) and shRNA for ADAR2 (shADAR2) along with a YFP construct for two days. After that, neurons were perfused with AMPA (30 μM) for 5 seconds. Two minutes after the first AMPA perfusion, neurons were subjected to a second AMPA perfusion with/without a general AMPAR antagonist GYKI (30 μM). [Ca2+]i was determined by single cells Fluo-3 fluorescence imaging as described under the Materials and Methods. B: The intensity of Fluo-3 fluorescence was quantified as described under the Materials and Methods. The results were calculated based on 75 cells. The results were the mean ± SEM from three independent assays. *p < 0.05.