Figure 8

Effects of ADAR2 over-expression or ADAR2 knock-down on TD-induced death of cortical neurons. A: Cortical neurons were transfected with wild-type ADAR2 construct or pcDNA6 vector along with YFP construct and treated with amprolium (Amp, 0 or 1 mmol/L) for 4 days. The cytotoxicity was determined by propidium iodide (PI) staining (top panel). The percentage of PI-positive neurons in culture treated with wild-type ADAR2 or pcDNA6 was quantified (bottom panel). The results were expressed as the mean ± SEM of three independent assays. * signifies statistical differences from the controls (p < 0.05). # signifies statistical differences from the cultures treated with TD and pcDNA6 (p < 0.05). B: Cortical neurons were transfected with shADAR2 or shCON and treated with amprolium (Amp, 0 or 1 mmol/L) for 4 days. The cytotoxicity was determined by PI staining (top panel). The percentage of PI-positive cells in cultures treated with shADAR2 or shCON was quantified (bottom). The results were expressed as the mean ± SEM of three independent assays. * signifies statistical differences from the controls (p < 0.05). # signifies statistical differences from the cultures treated with TD and shCON (p < 0.05).