Figure 4
From: α-synuclein induced synapse damage is enhanced by amyloid-β1-42
Aβ 1-42 enhanced the αSN-induced loss of synaptophysin. (A) The synaptophysin content of cortical neurons incubated for 24 hours with varying concentrations of αSN (●), αSN premixed with Aβ1-42 (50:1) (■) or αSN premixed with Aβ42-1 (50:1) (□). Values shown are the mean amount of synaptophysin (units) ± SD, n = 18. (*) = amount of synaptophysin significantly lower than in neurons incubated with the same concentration of αSN alone (P < 0.01). (B) The synaptophysin content of cortical neurons pre-treated for 1 hour with control medium (●) or 1 nM Aβ1-42 (○) and incubated for 24 hours with αSN as shown. Values shown are the mean amount of synaptophysin (units) ± SD, n = 12. (C) The synaptophysin content of cortical neurons incubated for 24 hours with αSN (●), αSN premixed with 7PA2-CM (■) or αSN premixed with CHO-CM (□). Values shown are the mean amount of synaptophysin (units) ± SD, n = 12. (*) = amount of synaptophysin significantly lower than in neurons incubated with the same concentration of αSN alone (P < 0.01).