SB and Sal exposure of primary neuronal cultures affects ER stress response, apoptosis and α-Syn aggregation. Primary neuronal cells from TG and NT mice, after 7 days in cultures, were treated with 10 mM SB or SB plus 20 μM Sal for 96 hs. Non-treated counterparts were included as controls (Con). Proteins extracted from different cultures were evaluated by Western blotting using antibodies to five ER stress markers, active caspase 3, α-Syn and GAPDH. Cells from TG mice expressed about 1.6 times (normalized to GAPDH) more α-Syn than those from NT controls. The SB-treated TG and NT cultures (TGSB & NTSB) expressed more abundant ER stress markers than their controls, and such stress response and caspase 3 activation are greater for the TG than NT cultures. These changes were reduced in cultures treated with SB plus Sal. The results are consistent with those obtained from the 3D5 cell line, which expresses higher α-Syn than primary neuronal cultures.