Figure 2
From: ER stress response plays an important role in aggregation of α-synuclein
SB-treated cultures accumulate α-Syn of different solubility and sizes. 3D5 cells were treated with 10 mM SB for 36 hs after 10 ds of differentiation and induced α-Syn expression. Cultures without the drug treatment were used as controls (Con). Total cell lysates (TL) were fractionated to obtain buffer-soluble (SN1), buffer-insoluble and salt plus sarkosyl-soluble (SN2) as well as sarkosyl-insoluble (SKI) fractions. These samples were evaluated by immunoblotting using TL:SN1:SN2:SKI at 1:1:3.75:9.37 loading and antibodies to α-Syn. GAPDH was used as loading control. Asterisk marks monomeric α-Syn.