Figure 3
From: ER stress response plays an important role in aggregation of α-synuclein
Accumulation of α-Syn and thioflavin-positive inclusions in SB-treated cultures. Retinoic acid-differentiated 3D5 cells with induced α-Syn expression (α-Syn+) or without (α-Syn-) were treated with SB (10 mM) or without (control) for 36 hs, followed by α-Syn immunofluorescence labeling and Thioflavin S staining. Alpha-Syn and Thioflavin S positive inclusions were detected only in the SB-treated cultures (marked by arrowheads). Nuclei were marked with DAPI stain. About 50% of cells with α-Syn expression and SB treatment contained aggregated α-Syn, and at the least 40% of such aggregates were Thioflavin S positive. Scale bar: 20 μm