Figure 5
From: ER stress response plays an important role in aggregation of α-synuclein
Ultrastructural evaluation of sarkosyl-insoluble preparations from cell cultures. (A-C) Sarkosyl-insoluble samples from SB-treated 3D5 cells depicted in Fig. 2 were adsorbed on EM grids and negatively stained with 5% uranyl acetate. They contain bundles of filamentous assemblies of diameter about 8-10 nm. The framed area in (B) is shown in (C) at higher magnification. (D) The filaments were decorated with 5-nm gold particles (arrows) by immunogold labeling using an anti-α-Syn antibody, thus verifying that they were assembled from α-Syn. Similar filamentous structures were not detected in cultures without the SB treatment (data not shown). Scale bar: 100 nm