Figure 9

Effects of SB and salubrinal on the survival of primary neuronal cultures. Primary neuronal cultures from TG and NT mice were exposed to 10 mM SB, 20 μM Sal, SB plus Sal for (A) 2 days and (B) 4 days, Non-treated counterparts were included as controls (Con). Without any drug treatment, cultures from TG and NT mice have a similar number of cells. A 2-d treatment of cultures with SB did not affect the survival of NT cultures, but resulted in a significant (*P < 0.05) cell loss in TG cultures when compared to untreated counterparts. The effect of SB on TG cell survival was significantly (#P < 0.05) blocked by co-treatment with Sal. Cell loss was noted in NT cultures treated with SB for 4 ds (**P < 0.01), but the extent of such change is significantly (#P < 0.05) less than that demonstrated in TG cultures with same duration of SB treatment. Co-treatment with Sal also reduced the cell loss caused by a 4d exposure to SB.