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Figure 3 | Molecular Neurodegeneration

Figure 3

From: Conformation dependent monoclonal antibodies distinguish different replicating strains or conformers of prefibrillar Aβ oligomers

Figure 3

Monoclonal antibodies distinguish conformational variants of Aβ PFOs. A. Aβ40 (40) and Aβ42 (42) were incubated under three different conditions: HEPES buffered saline pH7.4, water pH 2.5 or PBS pH 7.4. Monomer samples were spotted at time zero, while prefibrillar oligomers and fibrils were spotted at 72 and 240 hours of incubation respectively. All of the oligomer samples stain with A11, but M118 only reacts with the Aβ samples prepared at pH 2.5. B. Four different samples of prefibrillar oligomers were prepared in water pH 2.5 and stained with A11, M204 and M205. All samples stain with A11 (top row). Sample 1 stains with both 204 and 205 antibodies while sample 2 stains with only M204, sample 3 stains only with M205 and sample 4 stains with neither monoclonal antibody.

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Molecular Neurodegeneration

ISSN: 1750-1326