Figure 6

Paralysis in CL2006 with or without DAF-16, HSF-1, ACR-16 and UNC-38 knock down by RNAi. (A) Paralysis in CL2006 treated with or without JAY2-22-33 in C. elegans fed with vector control RNAi. Solid line, worms grown on bacteria containing empty vector alone; dotted line, worms grown on bacteria containing empty vector and treated with JAY2-22-33. (B) Paralysis in CL2006 treated with or without JAY2-22-33 in C. elegans fed with RNAi to down regulate DAF-16. Solid line, worms grown on daf-16 RNAi bacteria alone; dotted line, worms grown on daf-16 RNAi bacteria and treated with JAY2-22-33. (C) Paralysis in CL2006 treated with or without JAY2-22-33 in C. elegans fed with RNAi to down regulate HSF-1. Solid line, worms grown on hsf-1 RNAi bacteria alone; dotted line, worms grown on hsf-1 RNAi bacteria and treated with JAY2-22-33. (D) Paralysis in CL2006 treated with or without JAY2-22-33 in C. elegans fed with RNAi to down regulate ACR-16. Solid line, worms grown on acr-16 RNAi bacteria alone; dotted line, worms grown on acr-16 RNAi bacteria and treated with JAY2-22-33. (E) Paralysis in CL2006 treated with or without JAY2-22-33 in C. elegans fed with RNAi to down regulate UNC-38. Solid line, worms grown on unc-38 RNAi bacteria alone; dotted line, worms grown on unc-38 RNAi bacteria and treated with JAY2-22-33 (100 worms in each experiment, 3 independent experiments). n.s. = not significant