Figure 2
From: Identification of BACE1 cleavage sites in human voltage-gated sodium channel beta 2 subunit
Characterization of the major BACE1 cleavage site in an in vitro assay. A) Schematic representation of the Navβ2 (147ML/VI) and Navβ2 (147ML/AA) mutations introduced into the identified major BACE1 cleavage site. B) Cell-free generation of Navβ2-CTF in membranes from CHO cells expressing Navβ2 wildtype, Navβ2 (147ML/VI) or Navβ2 (147ML/AA). Treatment with the BACE inhibitor GL189 inhibited cleavage of wildtype Navβ2. Navβ2-CTF generation is nearly abolished in Navβ2 (147ML/VI) and Navβ2 (147ML/AA).