Figure 3
From: Identification of BACE1 cleavage sites in human voltage-gated sodium channel beta 2 subunit
Similar subcellular localizations of Navβ2 (147LM/VI) and Navβ2 (147LM/AA) as compared to wildtype Navβ2. A) Confocal images of enriched cultures expressing Navβ2 wildtype, Navβ2 (147ML/VI) or Navβ2 (147ML/AA) immunostained with a C-terminal V5 antibody. B) Western blot analysis of cells expressing either wildtype Navβ2 or Navβ2 (147LM/AA) subjected to sucrose gradient fractionation. Flotillin-1 staining marks lipid raft fractions. The Navβ2 (147ML/VI) and Navβ2 (147ML/AA) mutations in the BACE1 cleavage site did not visibly affect subcellular or lipid raft localization of Navβ2.