Localization pattern of exon-deletion mutants. Primary rat E18 hippocampal neurons were transfected with α-syn variant constructs. After 17 DIV, cells were fixed and immunostained for synapsin I. The left column shows the eGFP signal from the various α-syn constructs (green). The middle employs the "find edges" filter in Adobe Photoshop, which represents color transitions as lines. The right column shows a merge of the eGFP signal from the various α-syn constructs (green) with the synapsin I staining (red).