ASGFP transport behavior. (A) Time lapse fluorescent images of ASGFP transport particles in rat primary hippocampal neurons visualized at 10 DIV. The single-headed arrow indicates an ASGFP structure that moves along the axon, while the double-headed arrow indicates an ASGFP structure moving in the opposite direction. (B) Kymograph of the full time lapse series, with the time window depicted in A noted on top (46-81s). The particles are labeled as in panel A. One of the particles (double-headed arrow) is seen to move continuously in one direction, while other particle (single-headed arrow) moves in the opposite direction, pauses, and then bifurcates into stationary (s) and motile (m) particles.