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Figure 4 | Molecular Neurodegeneration

Figure 4

From: Amyloid beta dimers/trimers potently induce cofilin-actin rods that are inhibited by maintaining cofilin-phosphorylation

Figure 4

Numbers of rods induced by Aβd/t are highest in the dentate gyrus and mossy fiber tract. (A) Organotypic hippocampal slices were cultured for at least 8-10 days and were left untreated or treated with 1X Aβd/t, the same amount of the equivalent NC medium fraction, or scrambled Aβ peptide. After 48 h, slices were fixed and immunostained for cofilin and DNA (DAPI), and rods were quantified by counting with a 60x objective. Rod mapping from multiple slices onto a matrix grid of the hippocampus was performed as previously described using fiduciary markers from the stained nuclei layers to align hippocampal regions [29]. There were no differences detected in rod numbers or distribution between the untreated slices and those treated with NC medium or scrambled peptide and these were all combined to give the control panel. Rods induced by the Aβd/t were heavily concentrated over the dentate gyrus and mossy fiber tract (n = 18 for control slices and n = 12 for Aβd/t treated). (B) Rod quantification averaged per field over different regions of the slices. Each field acquired with the 60x objective has about 6-7 matrix grid squares. The only regions of significance (# = p = 0.05) for the rod numbers are in the dentate gyrus and mossy fiber tract.

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