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Table 1 Identification of phosphorylated tyrosines in tau by mass spectrometry after phosphorylation by Lck and Fyn

From: Tyrosine Phosphorylation of Tau by the Src Family Kinases Lck and Fyn

Phosphorylated Residue

Lck

Fyn

 

LC-MS/MS

a2D-MS/MS

LC-MS/MS

a2D-MS/MS

Tyr18

+

+

+

+

Tyr29

(ss)

-

(ss)

-

Tyr197

(ss)

+

(ss)

-

Tyr310

+

-

(ss)

-

Tyr394

+

-

+

-

  1. +, site positively identified by MS/MS
  2. (ss), survey scan (first MS spectrum) or MALDI-ToF MS; phosphopeptides identified by mass and mass-difference (phosphate loss) only.
  3. a LC-MS/MS analyses on phosphopeptides extracted from radiolabelled spots on 2D maps.