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Figure 4 | Molecular Neurodegeneration

Figure 4

From: Protein S blocks the extrinsic apoptotic cascade in tissue plasminogen activator/N-methyl D-aspartate-treated neurons via Tyro3-Akt-FKHRL1 signaling pathway

Figure 4

PS-mediated Akt phosphorylation of FKHRL1 suppresses FasL production and activation of caspase-8. (A) Expression of FKHRL1 and FKHRL1-TM (triple mutant) in mouse cortical neurons 24 h after adenoviral transduction determined by total FKHRL1 immunoblotting analysis. (B) Time-dependent survival (12-72 h) of neurons after transduction with Ad.FKHRL1-TM. Cell survival was quantified with a WST assay. (C) Western blots for FasL in whole-cell extracts from neurons transduced with Ad.FKHRL1-TM or control Ad.GFP and treated for 6 h with tPA/NMDA in the presence or absence of PS. Intensity of the FasL signal was normalized to β-actin. (D) Caspase-8 activity in neurons transduced with Ad.FKHRL1-TM or Ad.GFP and exposed to tPA/NMDA for 8 h in the presence or absence of PS. (E) Cell survival of neurons transduced with Ad.FKHRL1-TM or Ad.GFP and exposed to tPA/NMDA for 24 h in the presence or absence of PS. In all studies murine PS was used at 100 nM and was added simultaneously with tPA/NMDA. Mean ± SEM, n = 3 independent cultures in triplicate. NS, non-significant.

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Molecular Neurodegeneration

ISSN: 1750-1326