Figure 1

AAV2/1 mediated expression of mTNFα in the hippocampus of TgCRND8 mice leads to robust neuroinflammation. A-D. rAAV2/1-mTNFα or rAAV2/1-EGFP (Control) was injected into the hippocampus of 4 month old TgCRND8 mice and analyzed after 6 weeks. Representative images of GFAP immunoreactivity in paraffin embedded whole brain sections (A, B) and higher magnification of the hippocampus (C, D) of 5.5 month old TgCRND8 mice are shown. Scale Bar, 600 μm (A, B) and 25 μm (C, D). (n = 5/group). E-H. Representative images of Iba-1 immunoreactivity in paraffin embedded sections of 5.5 month old TgCRND8 mice injected with rAAV2/1 mTNFα or rAAV2/1-EGFP (Control). Whole brain sections (E, F) and the corresponding hippocampus (G, H) showing detailed morphology of the activated microglia is depicted. Scale Bar, 600 μm (E, F) and 25 μm (G, H). (n = 5/group). I-J. Quantitation of GFAP (I) and Iba-1(J) immunoreactivity burden (% area) in paraffin embedded sections of TgCRND8 mice expressing mTNFα or EGFP as control. The "positive pixel count" program available from Aperio was used for the analysis. (*p < 0.05; t test; n = 5/group).