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Figure 2 | Molecular Neurodegeneration

Figure 2

From: Hippocampal expression of murine TNFα results in attenuation of amyloid deposition in vivo

Figure 2

Reduction of hippocampal Aβ in AAV2/1-mTNFα expressing TgCRND8 mice. A-D. 4 month old TgCRND8 mice were stereotaxically injected in the hippocampus with either AAV2/1-mTNFα or AAV2/1-EGFP and sacrificed after 6 weeks (n = 5-6/group). Representative brain sections stained with 33.1.1 antibody (pan Aβ 1-16) depict attenuation of Aβ deposition in mTNFα expressing mice (C-D) compared to controls (A-B) in the immediate vicinity of the injection site. Scale Bar, 150 μm. E. Aβ plaque burden image analysis shows a significant decrease in amyloid deposition in mTNFα injected mice compared to control EGFP injected mice. At least three sections per sample, spaced 30 μm apart, were used for the analysis. (*p = 0.033; t test; n = 5/group). F. Quantitation of Congo Red stained compact Aβ plaques in mTNFα injected mice compared to control EGFP injected mice. Hippocampal compact plaques were counted from coronally dissected paraffin embedded representative brain sections of mTNFα and EGFP expressing mice. (*p = 0.05; t test; n = 5/group). G-H. Biochemical analyses of Aβ42 and Aβ40 levels by ELISA show significantly reduced SDS (G) and formic acid (H) extractable Aβ levels in mTNFα injected mice compared to controls (*p = 0.05; two way ANOVA; n = 4/group).

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