Figure 3
From: Hippocampal expression of murine TNFα results in attenuation of amyloid deposition in vivo
Analysis of Aβ42 and Aβ40 plaque burden reveals that acute focal expression of mTNFα in the hippocampus of TgCRND8 mice results in attenuation of Aβ deposition. A-F. 4 month old TgCRND8 mice were stereotaxically injected in the hippocampus with either rAAV2/1-mTNFα or rAAV2/1-EGFP and sacrificed after 6 weeks. Representative brain sections from EGFP and mTNFα expressing 5.5 month old mice as well as unmanipulated 4 month old mice were stained with anti Aβ42 antibody (A-C) and anti Aβ40 antibody (D-F). Insets depict representative magnified field of views from corresponding low power panel. Scale Bar, 600 μm (A-F); inset, 25 μm. (n = 5/group). G. Hippocampal Aβ42 and Aβ40 plaque burden analysis was performed in paraffin embedded brain sections of 5.5 month old mTNFα and EGFP expressing mice as well as unmanipulated 4 month old TgCRND8 mice. At least three sections per sample, spaced 30 μm apart, were used for the analysis. The "Positive Pixel Count" program available from Aperio was used for the analysis. (*p < 0.05; two way ANOVA; n = 5/group).