NSPCs express receptors for IFNγ. In a: Photomicrographs of proliferating NSPCs (NSPC-p) immunocytochemically labeled against Sox2 and nestin. In b: Photomicrographs of proliferating (NSPC-p) or differentiated (NSPC-d) NSPCs with indicated immunocytochemical markers are given showing that both receptors are expressed on individual cells. In c: real-time quantitative PCR with primers specific for IFNγ-R1 or IFNγ-R2 illustrate the expression of both IFNγ receptors on proliferating (NSPC-p) or differentiated (NSPC-d) NSPCs. Controls are spleen or total brain homogenates. Values are means +/- standard error of mean (SEM). Experiments were performed in triplicate and repeated independently at least three times.