Figure 2

IFNγ reduces the population extent of NSPCs. In a: Optical densities [9] correlate to the extent of NSPC populations after a 48-hour IFNγ treatment with indicated concentrations. Values are means +/- standard error of mean (SEM). Experiments were performed in triplicate and repeated independently at least three times. In b: Optical densities correlate to caspase 3/7 activity after a 48-hour IFNγ treatment with indicated concentrations. Values are means +/- standard error of mean (SEM). Experiments were performed in triplicate and repeated independently at least three times. In c: Photomicrographs of caspase 3/7 immunocytochemistries are given for proliferating (NSPC-p) NSPCs with or without IFNγ treatment. Beta Actin labeling visualizes cytoplasmatic structures to better correlate the caspase 3/7 signal to single cells. In d: Photomicrographs of BrdU labelings are given to visualize the amount of proliferating cells with or without IFNγ treatment. Additionally, quantification of BrdU⁺ cells is given. Values are means +/- standard error of mean (SEM). Experiments were performed in triplicate and repeated independently at least three times. DAPI⁺ nuclei are given in blue.