Figure 3

Cell type-specific marker expression after IFNγ treatment under proliferative conditions. In a: Results from real-time quantitative PCRs expressed as ΔΔCT (IFNγ- minus IFNγ+) of PBS-treated control and IFNγ-treated groups for the indicated markers of proliferating NSPCs. Higher values indicate a higher gene expression. Values are means +/- standard error of mean (SEM). Experiments were performed in triplicate and repeated independently at least three times. In b: Photomicrographs of proliferating NSPCs (NSPC-p) with indicated immunocytochemical markers (GFAP, βIII-tubulin, Map2a-c). Yellow arrows mark GFAP⁺/βIII-tubulin⁺ cells, the red arrow marks a GFAP^-/βIII-tubulin⁺ neuron and green arrows mark GFAP⁺/βIII-tubulin^- astrocytes. DAPI⁺ nuclei are given in blue. Diagrams show the percentages of immuno-positive cells from all DAPI⁺-cells with (IFNγ+) or without (IFNγ-) IFNγ treatment as indicated. Values are means +/- standard error of mean (SEM). Experiments were performed in triplicate and repeated independently at least three times.