Figure 4

Cell type-specific marker expression after IFNγ treatment during differentiation. In a: Photomicrographs of NSPC populations after a differentiation period of 7 days (NSPC-d) are given with indicated immunocytochemical markers and the corresponding quantification of immuno-positive cells from all DAPI⁺ cells with (IFNγ+) or without (IFNγ-) IFNγ treatment as indicated. Values are means +/- SEM. Experiments were performed in triplicate and repeated independently at least three times. In b: Photomicrographs of mouse embryonic stem cell-derived neural stem cells (NS cells) differentiated for 7 days (NSC-d) with indicated immunocytochemical markers with (IFNγ+) or without (IFNγ-) IFNγ treatment are given. Yellow arrows mark GFAP⁺/βIII-tubulin⁺ cells. Red arrows mark GFAP⁺/βIII-tubulin^- neurons and green arrows mark GFAP^-/βIII-tubulin⁺ astrocytes. Note the untypical morphology of GFAP⁺/βIII-tubulin⁺ cells under IFNγ treatment in comparison to control. Values are means +/- standard error of mean (SEM). Experiments were performed in triplicate and repeated independently at least three times.