Patch-clamp results of IFNγ treated NSPCs. In a: Voltage-step protocol used to probe cells for voltage-activated conductances (see current traces in panels b-d, g, and h). In b, c: GFAP+/βIII-tubulin+ cells typically exhibited an outward rectifying current voltage (IV) relationship (13/14 cells), IV-relationship was determined from non-leak-subtracted traces 10 ms after the voltage step (circle). In d: In 4/14 GFAP+/βIII-tubulin+ cells, voltage-gated inward currents were observed in addition to outward currents. In e: Image of cell filled with Alexa Fluor350 and later identified as GFAP+/βIII-tubulin+ (scale bar 30 μm, recordings illustrated in figure d) and f)). In f: None of the 14 GFAP+/βIII-tubulin+ cells exhibited action-potential-like events, upon depolarization to at least -30 mV evoked by current injections (inset). In g, h: Membrane currents typically observed in GFAP+/βIII-tubulin- cells (each recorded in 3/6 cells). In i: IV-relationships taken from non-leak-subtracted traces illustrated in g) (triangle) and h) (square).