Figure 3
From: 6-OHDA generated ROS induces DNA damage and p53- and PUMA-dependent cell death
Puma is required for 6-OHDA-induced loss of DA neurons in the SN and striatal DA depletion. C57 and Puma -/- mice were treated with 6-OHDA by unilateral intrastriatal injection and sacrificed one month later. A) 50 μM sections were stained for TH. Pictures are representative images of the SN ipsilateral to injection site. Locations of SN and ventral tegmental area (VTA) are indicated in the first panel. B) TH-positive cells were counted, ipsilateral to contralateral ratios were calculated and survival was expressed as a percentage of untreated control. Data were analyzed by two-way ANOVA (treatment: **, p < 0.01; genotype: **, p < 0.01). Results of Bonferroni post-tests are indicated on graph (***, p < 0.001). C) Striata were lysed and DA levels were analyzed by HPLC with electrochemical detection. Ipsilateral to contralateral ratios were calculated and expressed as a fraction of untreated control. Data were analyzed by two-way ANOVA (treatment: **, p < 0.01; genotype: ns). Results of Bonferroni post-tests are indicated on graph (*, p < 0.05).