Table 1 Primers for Reverse Transcription PCR
From: 6-OHDA generated ROS induces DNA damage and p53- and PUMA-dependent cell death
Gene | Primer 1 | Primer 2 |
|---|---|---|
18s rRNA | 5'-GGGAACGCGTGCATTTATCAG-3', | 5'-CGCTATTGGAGCTGGAATTAC-3' |
ATF3 | 5'-TGCCAAGTGTCGAAACAAGAA-3' | 5'-CGGTGCAGGTTGAGCATGTA-3' |
BiP | 5'-CTTCAATGATGCCCAGCGA-3' | 5'-CCAGGCCATATGCAATAGCAG-3' |
CHOP | 5'-TATCTCATCCCCAGGAAACG-3' | 5'-GATGTGCGTGTGACCTCTGT-3' |
GADD34 | 5'-GAGATTCCTCTAAAAGCTCGG-3' | 5'-CAGGGACCTGGACGGGCAGC-3' |
GAPDH | 5'-TGCCCCCATGTTTGTGATG-3' | 5'-TGTGGTCATGAGCCCTTCC-3' |
p58 IPK | 5'-TCCTGGTGGACCTGCAGTACG-3' | 5'-CTGCGAGTAATTTCTTCCCC-3' |
Puma | 5'-ACGACCTCAACGCGCAGTA-3' | 5'-CTAGTTGGGCTCCATTTCTGG-3' |
Xbp-1 | 5'-TAGAAAGAAAGCCCGGATGA-3' | 5'-CTCTGGGGAAGGACATTTGA-3' |