Figure 5

Passive immunization protects Tg2576 mice from memory deficits. Tg2576 mice at 13 months of age in three groups were studied: PBS-treated non-Tg mice, n = 14; PBS-treated Tg2576 mice, n = 10; 1A9-treated mice, n = 13; 2C3-treated mice, n = 12. Values indicate the mean ± SEM. (A) Y-maze test. Spontaneous alternation behavior during an 8-min session in the Y-maze task was measured in each group. Results of one-way ANOVA were as follows: F(3, 45) = 2.99, p < 0.05, **p < 0.05 vs PBS-treated non-Tg mice, #p < 0.05 vs PBS-treated Tg2576 mice. (B) Novel object recognition test. The retention session was carried out 24 h after the training. Exploratory preference during a 10-min session in the novel-object recognition test was measured in each group. Results of the two-way ANOVA were as follows: training/retention, F(1, 90) = 58.19, p < 0.01; animal group, F(3, 90) = 6.18, p < 0.01; interation of training/retention with animal group, F(3, 90) = 7.57, p < 0.01; **p < 0.01 vs corresponding trained mice. ##p < 0.01 vs PBS-treated non-Tg mice, **p < 0.01 vs PBS-treated Tg2576 mice. (C) Swimming-path length during a 60-s session in the water maze test was measured in each group. Results of the two-way ANOVA were as follows: trial, F(9, 450) = 25.51, p < 0.01; animal group, F(3, 450) = 14.85, p < 0.01; interaction of trial with animal group, F(27, 450) = 1.36, p = 0.11; **p < 0.01 vs PBS-treated non-Tg mice, #p < 0.05, ##p < 0.01 vs PBS-treated Tg2576 mice. Conditioned fear learning test: context-dependent (D) and cue-dependent (E) freezing times were measured. The results of one-way ANOVA were as follows: context-dependent test, F(3, 45) = 6.19, p < 0.01; cue-dependent test, F(3, 45) = 5.41, *p < 0.05; **p < 0.01 vs PBS-treated non-Tg mice, #p < 0.05, ##p < 0.01 vs PBS-treated Tg2576 mice.