Figure 6

Passive immunization protects Tg2576 mice from synaptic or neuronal degeneration. (A) Saline-insoluble, SDS-extractable synaptic proteins were examined by Western blot analysis and probed for PSD95 (1:250), drebrin (DB, 1:100), and synaptophysin (SYP, 1:2000). The p value was determined by one-way ANOVA: *p < 0.05. Representive Confocal immunofluorescence microscopy images of Tg2576 mouse brain. Sections of the cortex of the untreated (PBS)-, 1A9-treated, or 2C3-treatedTg2576 mouse brain were immunostained with PSD-95 (Upper part of panel A), Derbrin (DB, Middle part of panel A), synaptophysin (SYP, Lower part of panel A). Scale bar = 30 μm. (B) Confocal immunofluorescence microscopy images of Tg2576 mouse brain. Sections of the hippocampus of the untreated (PBS)-, 1A9-treated, or 2C3-treatedTg2576 mouse brain were immunostained with Fluoro Jade B (1:50000). Scale bar = 100 μm. (C) Representive Confocal immunofluorescence microscopy images of AD brain. Sections of the hippocampus of AD brains were immunostained with Fluoro Jade B. Scale bar = 20 μm.