Effect of METH on GLUT1 expression in hBEC. (A) Immunocytochemical expression of GLUT1 (green) merged with von Willebrand factor (vWF, red) and DAPI (blue) in hBECs. (B) Effect of METH (20 μM and 200 μM) on 55 kDa isoform GLUT1 protein levels in hBEC lysate protein with different co-treatments for 24 hr. (C) Effect of METH (20 μM) on 55 kDa isoform GLUT1 protein levels in hBEC lysate protein with different time periods and compared with 72 hr control cells. Both bar graphs show the results, which are expressed as ratio of GLUT1 to that of α-actin bands and presented as the mean values (± SEM; n = 5). *p < 0.05, **p < 0.01 statistically significant compared with controls. Scale bar indicates 40 μm in the panels of A.