METH inhibits glucose uptake in hBECs and animal model. (A-B) Regulation of GLUT1 levels by actinomycin D (Acd, transcription inhibitor, 100 ng/mL) and cycloheximide (Chx, translation inhibitor, 10 μg/mL) in hBECs exposed to METH for 24 hr. The results show the regulation of GLUT1 was affected at transcription level. Results are expressed as ratio of GLUT1 to that of α-actin, and presented as the mean values (± SEM; n = 3). (C) Effect of chronic METH administration (15 mg/kg body weight) on glucose uptake in vivo. *Statistically significant, *p < 0.05, **p < 0.01 compared with control.