Pyruvate protects glucose-deficient and METH induced cell dead. Confluent hBECs cultured in 96 well plates (20000 cells/well) in normal (+ glucose) or glucose-free (-glucose) media were exposed to 100 μM METH for 48 hr, and were analyzed for cell viability assay. Untreated in (+) glucose is a positive control and in (-) glucose is a negative control. Supplementation of pyruvate (PV) in glucose-free cell culture showed dose-dependent protection from METH and glucose deficiency. *Statistically significant (*p < 0.05, and ***p < 0.001) and #p < 0.05, ##p < 0.01 and ###p < 0.001 from (-) glucose+METH condition (fourth bar).