Figure 2
From: Generation and Characterization of a genetic zebrafish model of SMA carrying the human SMN2gene
Expression of hSMN2 RNA in transgenic zebrafish. Representative polyacrylamide gel showing RT-PCR product from hSMN2 RNA in transgenic lines. Lane 1, 1 kb DNA ladder. Lane 2, negative control from a wild-type nontransgenic fish. Lanes 3 and 4, samples from spinal cords of low and high SMN2 copy transgenic mice [3], respectively. Lanes 5-10, single 3 dpf Tg(hSMN2)os38 larva. Lanes 11-13, single 3 dpf Tg(hSMN2)os39 larva. Lane 14, single 3 dpf Tg(hSMN2)os40 larva. Lane 15, human breast cancer cell line as a control.