Figure 4From: Generation and Characterization of a genetic zebrafish model of SMA carrying the human SMN2geneDisrupting an intronic splice silencer site increases the amount of full-length hSMN RNA. (A) Representative polyacrylamide gel showing RT-PCR product obtained from hSMN RNA exons 4-8. One-cell Tg(hSMN2)os38 embryos were injected with 9, 15 or 18 ng of ISS-N1 MO. RNA samples were prepared at 3 dpf for RT PCR. (B) Quantification of the ratio of full-length hSMN/hSMNΔ7 RNA from three separate experiments shown as mean ± sd.Back to article page