Figure 5
From: Generation and Characterization of a genetic zebrafish model of SMA carrying the human SMN2gene
hSMN protein in Tg(hSMN2) larvae. (A) Representative western blot. Lane 1, 10 day old Smn-/-;SMN2+/+;delta7+/+mouse brain (10 μg); Lane 2-4, wt, Tg(hSMN2)os38, and Tg(hSMN2)os38 + 15 ng ISS-N1 MO zebrafish larvae collected at 3 dpf (150 μg of protein per lane). The blot was probed with human SMN [18] and β-actin monoclonal antibodies. (B) Quantification of hSMN band intensity from two experiments shown as mean ± sd.