Figure 7
From: Generation and Characterization of a genetic zebrafish model of SMA carrying the human SMN2gene
Tg(hSMN2 ) rescues the NMJ defect in smn mutants. Immunolabeling of presynaptic regions (SV2, red) and postsynaptic regions (α-bgt, green) of (A) 11 dpf WT larvae (n = 10); (B) 11 dpf smnY262stop-/-larvae (n = 13); (C) 11 dpf Tg(hSMN2)os38;smnY262stop-/-larvae (n = 12); (D) 14 dpf WT larvae (n = 8) (E) 14 dpf Tg(hSMN2)os38; smnY262stop-/-larvae (n = 12); Merge is the overlay. (F) The coefficients of co-localization were plotted and the means of each group were calculated. Tg(hSMN2)os38; smnY262stop-/-were significantly different compared to the smnY262stop-/-at 11 dpf (p < 0.0001). At 14 dpf, there was a significant difference between the Tg(hSMN2)os38;smnY262stop-/-and wild types. Scale bar, 80 μm.