Figure 2

Preferential expression of gigaxonin in neuronal tissues. Protein extracts (50 μg) of neuronal (A) and non neuronal (B) tissues from 24 week-old WT and GAN KO mice were immunoblotted with anti-gigaxonin (GigA) and anti-Actin antibodies. Cereb. = cerebellum; Sc. (C) = cervical section of spinal cord; Sc. (L) = lumbar section of spinal cord; SN = sciatic nerve. Specificity of detection is demonstrated by the absence of immunoreactivity in the tissues from KO mice. Note that the abundance of actin (comparable between WT and KO mice) varies in non neuronal tissues. The relative abundance of gigaxonin in multiple tissues was assessed by immunoblotting with GigA antibody (C, top panel). Coomassie blue staining indicates the equal loading of total proteins in all the tissues (C, bottom panel). (D) Temporal expression of gigaxonin, from embryonic stage E15 to adult stage (6 months) was determined in brain and lumbar sections of WT mice by immunoblotting with GigA, and normalized with anti-GAPDH antibody (n = 3; Mann-Whitney test, *, p < 0.05; bars represent standard deviation).