Lumbar motor neurons and axons are preserved in GAN mice. (A) Cross sections of lumbar spinal cord from 48-week-old WT and GAN mice, stained with VaCht (n = 4 animals). Inserts show lower magnification. (B-C) Cross sections of sciatic nerve (B), L5 dorsal and ventral roots (C) from 48-week-old WT and GAN mice, stained with toluidine blue. The numbers of distal axons (B), proximal motor and sensory axons (C) are represented for each mouse (n = 4 per genotype) and averaged (bar). Bars = 50 μm (A, B, C).