Figure 6

Increased abundance of NF subunits in the three GAN models. The relative increase of protein content was obtained by comparing the mean abundance in each of the three GAN models (KO1 = our GAN^ex3-5; KO2 = GAN^YY; KO3 = GAN^ex1) with WT mice (n = 3 mice per genotype, except n = 2 for 48 week-old KO2). (A) Expression levels were quantified in the brain, the lumbar section of spinal cord (Sc-L) and sciatic nerves (SN) by immunoblotting using anti-NFL, NFM and NFH antibodies and normalization with GAPDH antibody. (B) The relative abundance of the gigaxonin's partners MAP1B, MAP1S and TBCB was quantified using the corresponding antibodies, with a similar approach. (Mann-Whitney test, *, p < 0.05; bars represent standard deviation). The immunoblots corresponding to the abundance of the NF subunits and the gigaxonin's partners in brain of 48 week-old GAN models are represented in (C).