Figure 3
Dynein mutation reverts the systemic and molecular alterations associated with energy deficit in SOD1(G93A) mice. A- mRNA levels of lipoprotein lipase (LPL) in the epididimary white fat pad of wild type (+/+) and dynein mutant mice (Cra/+) bearing SOD1(G93A) transgene (SOD1m, black columns) or not (Wt, empty columns). *P < 0.05 versus Wt; #, p < 0.05 as indicated. Note that the SOD1(G93A)-associated decreased expression of LPL in the EPI is rescued by the dynein mutation in compound SOD1(G93A)/Cra mice. N = 9 mice per group. B- mRNA levels of fatty acid synthase (FAS), carnithine palmitoyl transferase 1A (liver form, CPT1A), Medium chain acyl CoA dehydrogenase (MCAD), peroxisome-proliferator activated receptor alpha (PPARα), peroxisome-proliferator activated receptor gamma co-activator 1 alpha (PGC1α), phosphoenolpyruvate carboxykinase (PEPCK) and peroxisome-proliferator activated receptor gamma (PPARγ) in the liver of the same mice than in A. *P < 0.05 versus Wt; #, p < 0.05 as indicated. Note that the SOD1(G93A)-associated decreased expression of FAS in the liver is rescued by the dynein mutation in compound SOD1(G93A)/Cra mice. N = 9 mice per group. C- Circulating triglycerides levels in the same mice than in A either in fed (right) or fasted conditions (left). Note that the SOD1(G93A) transgene leads to decreased fed triglycerides levels and that this is partially reverted in compound SOD1(G93A)/Cra mice. N = 9 mice per group. D- Circulating non-esterified fatty acids (NEFAs) levels in the same mice than in A in fasted conditions. Note that the SOD1(G93A) transgene leads to decreased fasted NEFAs levels and that this is fully reverted in compound SOD1(G93A)/Cra mice. N = 9 mice per group. E- mRNA levels of carnithine palmitoyl transferase 1B (muscle form, CPT1A), Medium chain acyl CoA dehydrogenase (MCAD), peroxisome-proliferator activated receptor gamma (PPARγ) and peroxisome-proliferator activated receptor gamma co-activator 1 alpha (PGC1α) in the gastrocnemius muscle of the same mice than in A. *P < 0.05 versus Wt. N = 9 mice per group.