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Figure 4 | Molecular Neurodegeneration

Figure 4

From: Lack of a-disintegrin-and-metalloproteinase ADAM10 leads to intracellular accumulation and loss of shedding of the cellular prion protein in vivo

Figure 4

No induction of p53 or p53-regulated pathways in ADAM10 cKO. (A) Western blot analysis in lysates of primary neurons from Prnp0/0, wt, tga20, ADAM10 cKO and littermate controls (all from E14 embryos) shows comparable protein amounts of p53. (B) Using quantitative RT-PCR, relative mRNA levels of p53 and its downstream signalling target Mdm2 were determined in brain homogenates of ADAM10 cKO mice and wildtype littermates controls at E14 (n = 4) and P1 (n = 3). No significant differences in expression levels of p53 and Mdm2 were observed between genotypes at both ages (E14: p = 0,75 for p53 and p = 0,44 for Mdm2; P1: p = 0,37 for p53 and p = 0,56 for Mdm2).

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Molecular Neurodegeneration

ISSN: 1750-1326