Figure 3
From: DJ-1 can inhibit microtubule associated protein 1 B formed aggregates
L166P mutation of DJ-1 decreased the ability of DJ-1 to inhibit the aggregation of MAP1b-LC. A MAP1b-LC was co-transfected with EGFP-DJ-1L166P or pEGFP-C into HEK293t cells for 48 hrs. Subsequently the cells were lysed and separated into Triton X-100 soluble and insoluble fractions. Western blot results showed that DJ-1L166P could not decrease the level of insoluble Flag-MAP1b-LC. B, MAP1b-LC was co-transfected with pEGFP-DJ-1L166P or pEGFP-C vector into SH-SY5Y cells for 48 hrs. Subsequently the cells were lysed and separated into Triton X-100 soluble and insoluble fractions. Western blot results showed that DJ-1L166P could not decrease the level of insoluble Flag-MAP1b-LC.