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Figure 4 | Molecular Neurodegeneration

Figure 4

From: Molecular interplay between leptin, insulin-like growth factor-1, and β-amyloid in organotypic slices from rabbit hippocampus

Figure 4

Effects of Aβ42 and leptin on the binding of STAT5 to IGF-1 promoter region. (a) Electrophoretic Mobility Shift Assay (EMSA) demonstrates that Aβ42 abrogates STAT5 binding to the exogenous oligonucleotide probes. Leptin treatment restores STAT5 binding to the oligonucleotide probe. (b) A Chromatin Immunoprecipitation (ChIP) assay demonstrates that treatment with leptin results in about 7-fold increase in STAT5 binding in the IGF-1 promoter region. ChIP analysis also revealed that treatment with Aβ42 attenuates binding of STAT5 to the IGF-1 promoter, while concomitant leptin treatment precludes this deleterious effect. ***p < 0.001 versus control; † † † p < 0.001 versus Aβ42.

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Molecular Neurodegeneration

ISSN: 1750-1326