Figure 5

IGF-1 reverses Aβ42-induced decrease in leptin expression levels. (a) Representative Western blot, (b) densitometric analysis, (c) ELISA immunoassay, and (d) Real time RT-PCR show that treatment of organotypic slices with Aβ42 for 72 hours significantly decreases leptin expression levels. IGF-1 treatment increases basal leptin levels and opposes the reduction induced by Aβ42 on leptin levels. *p < 0.05, **p < 0.01 and ***p < 0.001 versus control; ^† p < 0.05 and ^{† †} p < 0.01 versus Aβ42.