Altered synaptogenesis in IκBαM/M KI hippocampal neurons. A) Representative images of hippocampal neuronal cultures from WT and KI mice stained with a dendritic marker (MAP2/green) and either an excitatory presynaptic marker (VGLUT1/red) or an inhibitory presynaptic marker (VGAT/red). Area in white box is shown in higher detail to the right. Scale bar = 5 μm. B) Quantification of VGLUT1 and VGAT puncta density. N = 37-61 segments, * = p < 0.05, *** = p < 0.001. C) Representative images showing colocalization of presynaptic and postsynaptic markers in excitatory synapses (VGLUT1/PSD-95) and inhibitory synapses (GAD65/Gephyrin). Scale bar = 5 μm. D) Quantification showing percent of VGLUT1+ or GAD65+ puncta which also stain positive for PSD-95 or Gephyrin, respectively. N = 3 regions.