Figure 3

PKCδ dependent phosphorylation of PKD1 activation loop. N27 dopaminergic cells were treated with H₂O₂ (100 μM) with or without 1 μM rottlerin, and the lysates were probed for PKD1 activation phosphorylation (A). N27 dopaminergic cells were transfected with 1 μM PKCδ siRNA and non-specific siRNA and monitored for PKCδ protein expression and PKD1 activation loop phosphorylation after H₂O₂ treatment (B) and PKD1 kinase activity assay was performed. **, p < 0.01 denotes significant difference between NS-siRNA-H₂O₂ and PKCδ-siRNA-H₂O₂ groups (C).