Figure 4

Proteolytically activated PKCδ-CF contributes to PKD1 phosphorylation. N27 dopaminergic cells treated with H₂O₂ (100 μM) for 30, 60 or 90 minutes were monitored for PKCδ-CF (A). N27 dopaminergic cells were treated with H₂O₂ ± DEVD-fmk (50 μM) and ± ZVAD-fmk (50 μM and 100 μM) for 60 min and monitored for PKD1 activation and PKCδ cleavage (B). N27 dopaminergic cells stably expressing the cleavage-resistant mutant of PKCδ (PKCδ^D327A) were treated with H₂O₂ and monitored for PKD1 activation (C). PKD1 activation was monitored in N27 dopaminergic cells transfected with the catalytic fragment of PKCδ (PKCδ-CF) and the regulatory fragment of PKCδ (PKCδ-RF). Additionally, the mock transfection group treated with or without H₂O₂ was also monitored for PKD1 activation (D).