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Figure 5 | Molecular Neurodegeneration

Figure 5

From: Protein kinase D1 (PKD1) activation mediates a compensatory protective response during early stages of oxidative stress-induced neuronal degeneration

Figure 5

PKD1 has a cell survival function during oxidative stress in dopaminergic neuronal cells. N27 dopaminergic cells were transfected with 1 μM PKD1 siRNA and non-specific siRNA (A) and treated with 100 μM H2O2 for 120 minutes and monitored for cytotoxicity using Sytox green dye. Fluorescence measurements for the incorporation of Sytox green read using a flourescence plate reader (B) and visualised by phase contrast and fluorescence microscopy (C&D). DNA fragmentation assay (E) showed increased cytotoxicity and apoptosis in PKD1 knocked down samples exposed to H2O2. *, p < 0.05 and ***, p < 0.001 denote significant difference between Non-Specific siRNA- H2O2 and PKD1 siRNA-H2O2 treated groups.

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Molecular Neurodegeneration

ISSN: 1750-1326