PKD1 has a cell survival function during oxidative stress in dopaminergic neuronal cells. N27 dopaminergic cells were transfected with 1 μM PKD1 siRNA and non-specific siRNA (A) and treated with 100 μM H2O2 for 120 minutes and monitored for cytotoxicity using Sytox green dye. Fluorescence measurements for the incorporation of Sytox green read using a flourescence plate reader (B) and visualised by phase contrast and fluorescence microscopy (C&D). DNA fragmentation assay (E) showed increased cytotoxicity and apoptosis in PKD1 knocked down samples exposed to H2O2. *, p < 0.05 and ***, p < 0.001 denote significant difference between Non-Specific siRNA- H2O2 and PKD1 siRNA-H2O2 treated groups.