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Figure 7 | Molecular Neurodegeneration

Figure 7

From: Protein kinase D1 (PKD1) activation mediates a compensatory protective response during early stages of oxidative stress-induced neuronal degeneration

Figure 7

PKD1 activation acts as an early protective compensatory mechanism. A comparative time course graph based on quantifying PKD1 activation profile, PKCδ cleavage profile and cytotoxicity during H2O2 exposure (A). N27 dopaminergic cells transiently transfected with 5 μM full length PKD1 plasmid (PKD1WT) and 5 μM vector plasmid were treated with or without 100 μM H2O2 for 150 minutes and monitored for cytotoxicity using sytox green; PKD1 protected against cytotoxicity (B). N27 dopaminergic cells transiently transfected with 5 μM PKD1S916A plasmid and 5 μM vector plasmid were treated with or without 100 μM H2O2 for 150 minutes and monitored for cytotoxicity using sytox green; increased cytotoxicity was observed in the cells (C). N27 dopaminergic cells transiently transfected with 5 μM PKD1S744E/S748E and 5 μM vector plasmid (D) were treated with or without 100 μM H2O2 and monitored for cytotoxicity at various time points using sytox green. ***, p < 0.001 denotes significant difference between treatment groups from n≥6.

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