Reduced expression of macaque ERV-K-(HML-2) Gag in brains of BSE-infected macaques. (A). Extracts derived from the frontal/parietal cortex of BSE-infected (right) or non-infected (left) of Macaca fascicularis were subjected to PAGE, blotted to PVDF membrane and incubated with K2548. Immunoreactivity was visualized with NBT/BCIP. For loading control the blot was co-incubated with a monoclonal beta-actin antibody. (B). Densitometric analysis of A. NBT/BCIP-intensities of HERV-K-(HML-2) Gag were normalized to beta-actin intensities. Statistical analysis was performed using the unpaired students t-test (* p < 0.05). (C) Quantitative expression of ERV-K-(HML-2) assessed by QPCR. Expression was normalized to the housekeeping gene RPII. Points represent the mean of two experiments in duplicates. Statistical significance was determined by unpaired t-test (* p < 0.05).